Glutaminyl cyclases unfold glutamyl cyclase activity under mild acid conditions
نویسندگان
چکیده
منابع مشابه
The isoenzyme of glutaminyl cyclase is an important regulator of monocyte infiltration under inflammatory conditions
Acute and chronic inflammatory disorders are characterized by detrimental cytokine and chemokine expression. Frequently, the chemotactic activity of cytokines depends on a modified N-terminus of the polypeptide. Among those, the N-terminus of monocyte chemoattractant protein 1 (CCL2 and MCP-1) is modified to a pyroglutamate (pE-) residue protecting against degradation in vivo. Here, we show tha...
متن کاملSoluble Variants of Human Recombinant Glutaminyl Cyclase
Recombinant human Glutaminyl Cyclase expressed in E. coli is produced as inclusion bodies. Lack of glycosylation is the main origin of its accumulation in insoluble aggregates. Mutation of single isolated hydrophobic amino acids into negative amino acids was not able to circumvent inclusion bodies formation. On the contrary, substitution with carboxyl-terminal residues of two or three aromatic ...
متن کاملNucleotidyl Cyclase Activity of Particulate Guanylyl Cyclase A: Comparison with Particulate Guanylyl Cyclases E and F, Soluble Guanylyl Cyclase and Bacterial Adenylyl Cyclases Cyaa and Edema Factor
Guanylyl cyclases (GCs) regulate many physiological processes by catalyzing the synthesis of the second messenger cGMP. The GC family consists of seven particulate GCs (pGCs) and a nitric oxide-activated soluble GC (sGC). Rat sGC α1β1 possesses much broader substrate specificity than previously assumed. Moreover, the exotoxins CyaA from Bordetella pertussis and edema factor (EF) from Bacillus a...
متن کاملIn situ detection method for glutaminyl cyclase activity in polyacrylamide gels.
that achieved by TLC with numerous buffers (1,2,5) or by HV-TLE protocols (2) when multiple samples are analyzed. We have found that when 1 μL of a dye solution (1% orange G, 1% phenol red in pH 3.5 buffer) was electrophoresed in parallel with the phosphoamino acid standards, orange G migrated slightly ahead of pSer and phenol red migrated with pTyr, providing useful visible markers for electro...
متن کاملThe Isolation of Native Glutaminyl Cyclase from Saccharomyces cerevisiae
Glutaminyl Cyclase (QC)(2.5.2.3) catalyzes the cyclization of the N-terminal glutamine residues of peptides and proteins. Recent interest in QC has been generated by studies that have linked QC activity in human neuroendocrine tissue to diseases such as Alzheimer’s and osteoporosis. While QC has been isolated and characterized in multiple mammalian species, no attempt has been made to isolate t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 2004
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(04)00300-x